pGLO Lab
pGLO Observations , Data Recording & Analysis
1.
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Obtain your team plates. Observe your set of “+pGLO” plates under room light and with UV light. Record numbers of colonies and color of colonies. Fill in the table below.
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2.
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What two new traits do your transformed bacteria have?
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One of the new traits that our transformed bacteria had ampicillin resistance which was shown in the great number of colonies formed in the +pGLO LB/AMP. the other new trait was the neon glow that the +pGLO AMP/ARA had under the UV light.
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3.
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Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
I estimate that each colony has over a million bacteria because of how minuscule bacteria is. Since the plates had 27 to 34 colonies, I'm estimating that there was 27 million to 34 million bacteria spread on each plate. | |
4.
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What is the role of arabinose in the plates?
In the presence of arabinose, the GFP (Green Fluorescent Protein) turns on. The gene expression of GFP is shown when a UV light is shown over the bacteria and the colonies shine a bright, neon green fluorescence.
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5.
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List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
One current use for GFP is fusion tagging. Fusion tagging allows scientists to when and where a gene is expressed. Another use for GFP is biosensors. Biosensors detect lots of conditions within a cell including pH and ion concentration. A third current use for green fluorescent protein is in plasmids to see which cells have successfully taken up the plasmid.
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6.
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Give an example of another application of genetic engineering.
Another application of genetic engineering is medicine. Insulin and human growth hormone were both grown in the E.Coli bacteria. These were the first two medicines created from recombinant DNA tech, and since then, there have been many more products released.
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