Candy Electrophoresis Lab
1). Some of our dye bands expanded drastically. The shades of the colors of the reference dye to our dyes were slightly different. I think that this happened because the dye from the candies probably also have a lot of other ingredients while the sample dyes do not. Some of the dyes could have possibly gone in the "wrong" direction, but since we had to go to the assembly during our lab, we are not quite sure.
2). I think that Red 40 would migrate very similarly to Citrus Red 2. I believe this because they are almost the same exact length. Since the rate of the migration is based on length and they are close to the same length, then they will migrate very similarly to each other.
3). I think that the reason dog food manufacturers put artificial food colors in dog food to make it look more like the food it is supposed to taste like. For example, a dog food that tastes like chicken will probably have a chicken-like color to it. If the dog food had no food dye added to it and was a bland grey color, dogs would not eat it even if it had the same exact taste as with the food dye.
4). A reason that artificial food colors may be preferable to natural food dyes is because it can create more colors than natural food dyes can and it also may be cheaper to make for the food companies than natural food dye.
5). One factor that controls the distance that the colored dye solutions migrate are the length of the certain dye. The other factor that controls the distance is the electrical current which attracts, or repels, the dyes to go towards the other end of the gel.
6). The force that helps move the dyes through the gel is the electrical current.
7). The component of the electrophoresis system that causes the molecules to separate by size is the gel. The gel acts as a filter. The molecules with smaller lengths will go through the gel faster than the bigger molecules because they can go through the gel more easily. The opposite is true for the bigger molecules which take longer to pass through and this would be shown in the gel.
8). I expect that the DNA molecules would be separated by weight. I believe that the lighter molecules will travel faster than the heavier molecules through the gel. Because of this, I expect the molecule with 600 daltons to travel the farthest away from the gel. The next farthest molecule would be 1000, then 2000. And then finally the molecule that weighs 5000 daltons would be closest to the starting point.
2). I think that Red 40 would migrate very similarly to Citrus Red 2. I believe this because they are almost the same exact length. Since the rate of the migration is based on length and they are close to the same length, then they will migrate very similarly to each other.
3). I think that the reason dog food manufacturers put artificial food colors in dog food to make it look more like the food it is supposed to taste like. For example, a dog food that tastes like chicken will probably have a chicken-like color to it. If the dog food had no food dye added to it and was a bland grey color, dogs would not eat it even if it had the same exact taste as with the food dye.
5). One factor that controls the distance that the colored dye solutions migrate are the length of the certain dye. The other factor that controls the distance is the electrical current which attracts, or repels, the dyes to go towards the other end of the gel.
6). The force that helps move the dyes through the gel is the electrical current.
7). The component of the electrophoresis system that causes the molecules to separate by size is the gel. The gel acts as a filter. The molecules with smaller lengths will go through the gel faster than the bigger molecules because they can go through the gel more easily. The opposite is true for the bigger molecules which take longer to pass through and this would be shown in the gel.
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